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Neurons were transfected with mRFP-P-actin on day in vitro 6 (DrV6) using LipofectAMINE™ 2000 (Invitrogen) according to the manufacturer's protocol

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An enrichment of RTC-specific T cells was observed at recruitment (prior to exposure) among individuals who went on to have an abortive infection relative to those who had a detectable infection